STUDIES

Please reach out if you are interested in performing any research or clinical studies regarding TuttleNumbNow. We are happy to help in any way. 

·         The TNN anesthesia protocol achieved profound anesthesia in 94.2% of mandibular first molar cases without requiring IANB, demonstrating its effectiveness as a primary anesthetic strategy

o    Gregory K. Tuttle, Carter K. Newell, LaDawn Boucher, Jill Taylor, and Jared J. Tuttle,

o    Efficacy of the TuttleNumbNow anesthesia protocol for mandibular first molars: a retrospective study,

o    JDAPM Volume 26, Issue 2 (April 1, 2026)

o    https://jdapm.org/DOIx.php?id=10.17245/jdapm.2026.26.2.133 

·         TuttleNumbNow as a new primary IO injection technique

o    Tyler Dolphin, Sara Fowler, Melissa Drum, John Nusstei, Al Reader, John Draper

o    Efficacy of the TuttleNumbNow Intraosseous Method for Pulpal Anesthesia in the Mandibular First Molar: A Prospective, Randomized, Crossover Study

o    2019 Feb;23(2):673-680. doi: 10.1007/s00784-018-2481-3. Epub 2018 May 10.

o    https://pubmed.ncbi.nlm.nih.gov/38266911/

o    Letter to the Editor: https://pubmed.ncbi.nlm.nih.gov/38880583/

• Needle Bending Safety Study
• Tuttle JJ, Davidson AD, Tuttle GK.
• Bending 30-gauge needles using a needle guide: fatigue life evaluation.
• J Dent Anesth Pain Med. 2023 Oct;23(5):281-285. 
• https://jdapm.org/DOIx.php?id=10.17245/jdapm.2023.23.5.281

• Intraosseous (IO) injections allows placement of local anesthetic solution directly into the cancellous bone adjacent to the tooth to be anesthetized
• Reader, Alfred, John Nusstein, and Melissa Drum.
• Successful local anesthesia for restorative dentistry and endodontics.
• Quintessence Publishing Company Incorporated, 2017.p.132
• https://pubmed.ncbi.nlm.nih.gov/38266911/

• Scalpel Designed Bevel Studies- a significant lower pain level was found in Scalpel Bevel
• M. Dau, I. Buttchereit, C. Ganz, B. Frerich, E.N. Anisimova, M. Daubländer, P.W. Kämmerer,
• Influence of needle bevel design on injection pain and needle deformation in dental local infiltration anaesthesia – randomized clinical trial
• International Journal of Oral and Maxillofacial Surgery,Volume 46, Issue 11,2017,Pages 1484-1489ISSN 0901-5027
• https://www.sciencedirect.com/science/article/abs/pii/S0901502717315217

• 50 years since new local anesthesia technique was invented by Gow Gates with an alternative to the IA Nerve Block
• Isen, David. "FEATURES-PRODUCT PROFILE-
• A New Method for Intraosseus Injection: The X-Tip."
• Oral Health 90.7 (2000): 44-46.
• https://www.oralhealthgroup.com/features/product-profile-a-new-method-for-intraosseus-injection-the-x-tip/
  
  
• IO anesthesia is safe to use as a primary technique in teeth with normal pulp.
• The effect of intraosseous local anesthesia of 4% articaine with 1:100,000 epinephrine on pulpal blood flow and pulpal anesthesia of mandibular molars and canines
• Kadkao Vongsavan, Tshering Samdrup, Kanittha Kijsamanmith, Praphasri Rirattanapong, Noppakun Vongsavan
• Clin Oral Investig, 2019 Feb;23(2):673-680., doi: 10.1007/s00784-018-2481-3. Epub 2018 May 10.

• For a “hot tooth” with symptomatic irreversible pulpitis (SIP), an IAN is only 64% effective
• Reader A, Nusstein J, Drum M. Successful Local Anesthesia for Restorative Dentistry and Endodontics, Quintessence Publishing Co, Inc. Hanover Park, IL. 2011, p 34, 133, 134, 135, 138, 139, 140, 159.
• https://www.aae.org/specialty/successful-local-anesthesia-what-endodontists-need-to-know/
• Reader, Successful Local Anesthesia: What Endodontists Need to Know, 2017
  
  
• The IAN statistically falls short across multiple medical disciplines; advantages of finding an alternative simple technique
  
  
• Thangavelu, K., R. Kannan, and N. Senthil Kumar. "Inferior alveolar nerve block: Alternative technique." Anesthesia, essays and researches 6.1 (2012): 53.
• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4173425/

• Needle Gauge Length
• Webb, Laura J. "Orabloc®."
• https://www.orabloc.com/wp-content/uploads/Tip-Selecting-the-right-needle.pdf

• Increased Anesthetic Requirement in Individuals with Red Hair: Phenotypic and Genotypic Correlation
• Red hair appears to be a distinct phenotype linked to anesthetic requirement in humans that can also be traced to a specific genotype.
• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1362956/

• TuttleNumbNow Evaluation
• Anesthetic Efficacy of TuttleNumbNow Intraosseous Injection vs Inferior Alveolar Nerve Block Injection on Mandibular First Molars
• Christopher Smith, Susan Paurazas, Maha Ahmad, Mazin Askar
• Pending publication